近日，农业农村部沼气科学研究所2019级巴基斯坦籍留学生Ayesha Nisar以第一作者在Frontiers in Microbiology (JCR-Q1, IF=5.640)上发表了题为“Genome-Wide Analyses of Proteome and Acetylome in Zymomonas mobilis Under N2-Fixing Condition”的文章。本研究报道了运动发酵单胞菌在固氮培养条件下的蛋白质组学和首个乙酰化修饰蛋白质组学分析，揭示了蛋白质乙酰化修饰可能调控运动发酵单胞菌的多个代谢途径。农业农村部沼气科学研究所秸秆资源化利用创新团队吴波副研究员为本文通讯作者。
Recently, a GSCAAS Master’s student Ayesha Nisar at Biogas Institute of Ministry of Agriculture and Rural Affairs, CAAS, published a research article entitled “Genome-Wide Analyses of Proteome and Acetylome in Zymomonas mobilis Under N2-Fixing Condition” in Frontiers in Microbiology (JCR-Q1, IF=5.640). In this paper, Ayesha Nisar is the first author, while Associate Prof. Dr. Bo Wu at Biogas Institute of Ministry of Agriculture and Rural Affairs is the corresponding author. In this study, authors reported the analysis of both genome-wide proteome and the first acetylome of Zymomonas mobilis under the N2-fixing condition, and revealed that protein acetylation likely regulatesvarious metabolic pathways in Z. mobilis.
原文链接Full-text link:
https://www.frontiersin.org/articles/10.3389/fmicb.2021.740555/full#h1
https://doi.org/10.3389/fmicb.2021.740555
运动发酵单胞菌是一种固氮产乙醇细菌，广泛用于生物乙醇生产和其他生物转化过程。有趣的是，在固氮条件下，它有利于乙醇生产而不是生物量积累。因此，通过生物固氮并将其用作氮源，而不是依赖额外供应的化学氮源，可以显著节省生物乙醇工业的成本。
此外，运动发酵单胞菌的固氮过程似乎与增加的葡萄糖-乙醇转化率和降低的葡萄糖-生物量比率相关联，这种现象更加凸显解析固氮调控机制的重要性。然而目前对运动发酵单胞菌的固氮调控知之甚少，因此研究者对固氮条件下运动发酵单胞菌中的蛋白质组和蛋白质乙酰化修饰进行了高通量分析，并建立了它的第一个乙酰化修饰蛋白组。蛋白质组学分析结果显示，上调的蛋白质主要富集在固氮、运动、趋化、鞭毛组装、能量产生、运输和氧化还原过程，而下调的蛋白质主要与能量消耗和生物合成过程有关。乙酰化修饰蛋白质组学分析揭示了固氮条件下197种独特的乙酰化修饰蛋白质，富集在固氮、中心碳代谢、氨同化、蛋白质生物合成和氨基酸代谢等主要代谢途径中。进一步分析揭示了糖酵解酶、固氮酶复合体及其主要调控因子NifA，以及 GS/GOGAT 循环中各种酶的乙酰化修饰。该研究首次证实了运动发酵单胞菌的蛋白质广泛存在乙酰化翻译后修饰，暗示蛋白质乙酰化修饰可能在运动发酵单胞菌代谢中发挥重要调控功能。
Z.mobilis is a nitrogen-fixing ethanologenic bacterium that is widely used in bio-ethanol production and other bioconversion processes. Interestingly, it favors ethanol production over biomass accumulation under the nitrogen-fixing condition. Thus, it enables significant savings for the bioethanol industry by fixing atmospheric nitrogen as a nitrogen source instead of relying on additional chemical nitrogen source supply.
Moreover, nitrogen fixation in Z. mobilis appears to be coupled with increased glucose-to-ethanol turnover and reduced glucose-to-biomass ratios, emphasizing the importance of deciphering regulatory mechanisms underlying the nitrogen fixation. However, little is known about the regulation of nitrogen fixation in Z. mobilis. Researchers herein conducted a high throughput analysis of proteome and protein acetylation in Z. mobilis under N2-fixing conditions and established its firstacetylome. The proteomic analysis revealed that the upregulated proteins were mainly enriched in processes of nitrogen fixation, motility, chemotaxis, flagellar assembly, energy production, transportation, and oxidation–reduction. Whereas, downregulated proteins are mainly related to energy-consuming and biosynthetic processes. The acetylome analyses revealed, under the N2-fixing condition, 197 uniquely acetylated proteins that were enriched in major pathways such as nitrogen fixation, central carbon metabolism, ammonia assimilation pathway, protein biosynthesis, and amino acid metabolism. The further analysis revealed acetylation in glycolytic enzymes of central carbon metabolism, the nitrogenase complex and its master regulator NifA, and the enzyme in GS/GOGAT cycle. This research for the first time demonstrated the universal acetylation modification of Z. mobilis proteins, suggesting that protein acetylation likely plays an important role in metabolic regulation in Z. mobilis.
图1.固氮条件下运动发酵单胞菌的蛋白质组学分析
Figure 1. Proteomics analyses of Zymomonas mobilis under N2-fixing conditions
图 2.固氮条件下运动发酵单胞菌中差异表达蛋白质的GO 富集分析
Figure 2. GO terms enrichment analysis of differentially expressed proteins in Z. mobilis under N2-fixing condition
图 3. 固氮条件下运动发酵单胞菌差异表达蛋白质的KEGG通路富集分析
Figure 3. KEGG pathways enrichment analysis of differentially expressed proteins in Z. mobilis under N2-fixing condition
图 4. 固氮和非固氮条件下运动发酵单胞菌的蛋白质乙酰化修饰
Figure 4. Differential protein acetylation in Z. mobilis under N2-fixing and non-fixing conditions
图 5. 固氮和非固氮条件下运动发酵单胞菌中差异乙酰化蛋白质的富集分析
Figure 5. Enrichment analysis of differentially acetylated proteins in Z. mobilis under N2-fixing and non-fixing conditions
图 6. 固氮(A)和非固氮条件下(B)运动发酵单胞菌中差异乙酰化蛋白质相互作用网络
Figure 6. Protein interaction networks of differentially acetylated proteins in Z. mobilis under (A) N2-fixing condition and (B) non-fixing conditions
图 7. 运动发酵单胞菌中心碳代谢和固氮代谢途径中蛋白质的调控和乙酰化修饰
Figure 7. Regulation and acetylation of proteins in metabolic pathways of central carbon metabolism and nitrogen fixation in Z. mobilis in the present study
该研究得到国家自然科学基金（32070036）、中央公益性科研机构基础研究基金（Y2019LM02）和国家农业科技中心地方财政基金（NASC2020AR02）的支持。
The study was supported by the National Natural Science Foundation of China (32070036), the Central Public-interest Scientific Institution Basal Research Fund (Y2019LM02), and the Local financial funds of National Agricultural Science and Technology Center (NASC2020AR02).
作者简介：
Ayesha Nisar为2019年春季入学的巴基斯坦籍留学生，目前在农业农村部农业农村部沼气研究所攻读生物化学与分子生物学硕士研究生，导师为吴波副研究员。迄今为止，她以第一作者在Frontiers in Microbiology (IF=5.640)、Microbiological Research (IF=5.415)、Mitochondrial DNA A (IF=1.514)等SCI期刊发表论文3篇。另有一篇以她为第一作者的论文正处于 SCI 期刊 Science of the Total Environment (IF=7.963) 修订阶段。此外，Ayesha 还在硕士期间与他人合作在Veterinary Research (IF=3.683)、Mitochondrial DNA A (IF=1.514)、Genes (IF=4.096)、Mitochondrion (IF=4.16)和Parasitology International (IF=2.23)等SCI期刊发表多篇学术论文。其累计影响因子达到 36.215。
Ayesha Nisar from Pakistan, is currently in her final year of Master Program (of 2019 Spring Semester batch) at Biogas Institute of Ministry of Agriculture and Rural Affairs, CAAS, Chengdu, under the supervision of Associate Prof. Dr. Bo Wu. So far, she has published 3 research articles as a first author in well-reputed SCI Journals, such as Frontiers in Microbiology (IF=5.640), Microbiological Research (IF=5.415), and Mitochondrial DNA A (IF=1.514). While one of her research article as a first author is at revision stage in SCI journal Science of the Total Environment (IF=7.963). Ayesha also co-authored several SCI publications during her Master’s, such as articles in Veterinary Research (IF=3.683), Mitochondrial DNA A (IF=1.514), Genes (IF=4.096), Mitochondrion (IF=4.16), and Parasitology International (IF=2.23). Her accumulative impact factor is 36.215.
吴波副研究员简介及著作列表：
Associate Prof. Dr. Bo Wu’sprofile and Publications list:
https://www.researchgate.net/profile/Bo-Wu-72
Ayesha Nisar发表论文列表：
Ayesha Nisar’s Publications:
https://www.researchgate.net/profile/Ayesha-Nisar-2/research
Duringher lab work
With supervisor and lab members
Team BIOMA
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